Immunochemical characterization of the Rh CW antigen using human monoclonal antibodies

Vox Sang. 1997;73(3):174-81. doi: 10.1046/j.1423-0410.1997.7330174.x.

Abstract

Background and objectives: Human monoclonal antibodies have been produced against various Rh antigens. We report here the production of another one against Rh C and the first such antibody against Rh CW.

Materials and methods: Two heterohybridomas secreting IgG human monoclonal antibodies against Rh CW and Rh C (MS-353 and MS-242 respectively) were produced from alloimmunized donors. Their specificity was confirmed by serological testing.

Results: MS-353 reacted with all CW-positive phenotypes but not with any CW-negative phenotypes, whereas MS-242 reacted with all CW-positive and C-positive phenotypes. Using 125I-labelled antibodies, the average number of CW sites/red cell was 32,000 (R1WR1W, 15,200 (R1WR1), 19,800 (R1WR2), 15,300 (R1Wr), 26,200 (r'Wr), and the average number of C sites per red cell on equivalent CW/C-positive phenotypes was similar: 22,400 (R1R1), 21,800 (R1WR1), 12,300 (R1R2), 11,700 (R1WR2), 13,400 (R1r), 15,100 (R1Wr), 21,100 (r'r), 18,700 (r'Wr). MS-353 and MS-242 were mutually inhibitory. Both antibodies specifically immunoprecipitated a 33- to 34-kD polypeptide from 125I-surface-labelled cells.

Conclusion: MS-353 is suitable for the serological detection of CW-positive cells, and the immunochemical data are entirely consistent with the report that the CW antigen is associated with a point mutation in the RHCE gene [Blood, 1995;86:1196-1201].

MeSH terms

  • Antibodies, Monoclonal
  • Antibody Specificity
  • Humans
  • Immunochemistry
  • Isoantigens / blood*
  • Precipitin Tests
  • Regression Analysis
  • Rh-Hr Blood-Group System / immunology*

Substances

  • Antibodies, Monoclonal
  • Isoantigens
  • Rh-Hr Blood-Group System