Temporal brain imaging by a rapid scan ESR-CT system in rats receiving intraperitoneal injection of a methyl ester nitroxide radical

Magn Reson Imaging. 1997;15(9):1079-84. doi: 10.1016/s0730-725x(97)00136-7.

Abstract

We performed in vivo ESR-CT (electron spin resonance-computed tomography) on rats' heads, in which the blood-brain barrier-permeable nitroxide radical, 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (PCAM) was injected intraperitoneally, using a rapid scan ESR-CT system operating at 700 MHz. In a spatiotemporal study we found that different regions of the brain showed differences in the rate of decay of the radical. Repeated injection of PCAM gave clear ESR-CT images of the brain. We think that the present method is useful for evaluating the capacity to eliminate exogenous free radicals in some parts of the brain.

MeSH terms

  • Animals
  • Brain / anatomy & histology*
  • Brain / diagnostic imaging
  • Cyclic N-Oxides* / metabolism
  • Electron Spin Resonance Spectroscopy*
  • Head / anatomy & histology*
  • Head / diagnostic imaging
  • Injections, Intraperitoneal
  • Male
  • Pyrrolidines* / metabolism
  • Radiation-Protective Agents* / metabolism
  • Rats
  • Rats, Wistar
  • Spin Labels
  • Tomography, X-Ray Computed*

Substances

  • Cyclic N-Oxides
  • Pyrrolidines
  • Radiation-Protective Agents
  • Spin Labels
  • 3-carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyl-N-oxyl