Genomic cis-regulatory logic: experimental and computational analysis of a sea urchin gene

Science. 1998 Mar 20;279(5358):1896-902. doi: 10.1126/science.279.5358.1896.

Abstract

The genomic regulatory network that controls gene expression ultimately determines form and function in each species. The operational nature of the regulatory programming specified in cis-regulatory DNA sequence was determined from a detailed functional analysis of a sea urchin control element that directs the expression of a gene in the endoderm during development. Spatial expression and repression, and the changing rate of transcription of this gene, are mediated by a complex and extended cis-regulatory system. The system may be typical of developmental cis-regulatory apparatus. All of its activities are integrated in the proximal element, which contains seven target sites for DNA binding proteins. A quantitative computational model of this regulatory element was constructed that explicitly reveals the logical interrelations hard-wired into the DNA.

Publication types

  • Comment
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / physiology
  • Computer Simulation
  • DNA-Binding Proteins / metabolism
  • Embryo, Nonmammalian / metabolism
  • Endoderm / metabolism
  • Gastrula / metabolism
  • Gene Expression Regulation, Developmental*
  • Lithium Chloride / pharmacology
  • Models, Genetic
  • Molecular Sequence Data
  • Mutagenesis
  • Promoter Regions, Genetic / genetics
  • Promoter Regions, Genetic / physiology*
  • Proteins / genetics*
  • Proteins / physiology
  • Sea Urchins / embryology
  • Sea Urchins / genetics*
  • Sea Urchins / metabolism
  • Transcription, Genetic* / drug effects

Substances

  • Cell Adhesion Molecules
  • DNA-Binding Proteins
  • Proteins
  • endo16 protein, sea urchin
  • Lithium Chloride