Expression and function of Trk-C in favourable human neuroblastomas

Eur J Cancer. 1997 Oct;33(12):2054-7. doi: 10.1016/s0959-8049(97)00309-2.

Abstract

Human neuroblastomas express the neurotrophin receptors trk-A and trk-B. Favourable outcome is associated with expression of trk-A, while unfavourable, MYCN amplified tumours express trk-B. In this study we examined the expression of trk-C in primary neuroblastoma tumour-derived cell lines. We found by Northern blot analysis that trk-C mRNA is expressed in 14 of 55 (25%) primary tumours. Trk-C was expressed in significantly more lower stage tumours (stage 1, 2, 4S) than higher stage tumours (stage 3, 4, P < 0.04). The expression of trk-C was correlated positively with survival and negatively correlated with MYCN amplification. We also studied the function of trk-C in transfected cell lines and found that NT-3 promotes both cell survival and differentiation. Our results suggest that trk-C is involved in the biology of favourable neuroblastomas.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Northern
  • Gene Amplification
  • Genes, myc / genetics
  • Humans
  • Neoplasm Proteins / metabolism*
  • Neoplasm Staging
  • Nerve Growth Factors / metabolism
  • Nerve Growth Factors / physiology
  • Neuroblastoma / metabolism*
  • Neuroblastoma / pathology
  • Neurotrophin 3
  • Polymerase Chain Reaction
  • Receptor Protein-Tyrosine Kinases / metabolism*
  • Receptor, trkC
  • Receptors, Nerve Growth Factor / metabolism*
  • Survival Analysis
  • Tumor Cells, Cultured

Substances

  • Neoplasm Proteins
  • Nerve Growth Factors
  • Neurotrophin 3
  • Receptors, Nerve Growth Factor
  • Receptor Protein-Tyrosine Kinases
  • Receptor, trkC