t(2;5) positive lymphoma with peripheral blood involvement

Leuk Lymphoma. 1998 Jan;28(3-4):415-22. doi: 10.3109/10428199809092698.

Abstract

We report two cases of CD30 and t(2;5) positive lymphomas with peripheral blood (PB) involvement. Case one demonstrated the histological appearance of a diffuse large cell lymphoma with disease in the bone marrow (BM) and PB. Immunoperoxidase stains of the BM for CD30 proved to be of value in detecting disease. RT-PCR for the t(2;5) translocation product was positive in the PB, BM and lymph node. Case two had a typical anaplastic large cell lymphoma (ALCL) morphology, with a suboptimal BM biopsy, but abnormal circulating cells in the PB showing the presence of the NPM/ALK fusion product demonstrated by RT-PCR. The first case demonstrates that not all CD30 positive and t(2;5)-associated lymphomas have an anaplastic appearance. Routine staining for CD30 and EMA in BM biopsies is useful for pathological staging. The significance of the t(2;5) in defining a specific histological subtype is unclear. RT-PCR for the t(2;5) is a more sensitive test to detect disease in PB and BM as compared with light microscopy. The clinical significance of molecular staging for patients with ALCL using RT-PCR needs to be evaluated.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Chromosomes, Human, Pair 2*
  • Chromosomes, Human, Pair 5*
  • Humans
  • Immunoenzyme Techniques
  • Ki-1 Antigen / analysis
  • Lymph Nodes / pathology
  • Lymphoma, Large-Cell, Anaplastic / genetics*
  • Lymphoma, Large-Cell, Anaplastic / pathology
  • Male
  • Polymerase Chain Reaction
  • Translocation, Genetic

Substances

  • Ki-1 Antigen