Electrospray ionization and matrix assisted laser desorption/ionization mass spectrometry: powerful analytical tools in recombinant protein chemistry

Nat Biotechnol. 1996 Apr;14(4):449-57. doi: 10.1038/nbt0496-449.

Abstract

Electrospray ionization and matrix assisted laser desorption/ionization are effective ionization methods for mass spectrometry of biomolecules. Here we describe the capabilities of these methods for peptide and protein characterization in biotechnology. An integrated analytical strategy is presented encompassing protein characterization prior to and after cloning of the corresponding gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biotechnology
  • Mass Spectrometry / methods*
  • Molecular Weight
  • Oligonucleotide Probes / chemical synthesis
  • Oligonucleotide Probes / chemistry
  • Oligonucleotide Probes / genetics
  • Peptide Mapping
  • Protein Conformation
  • Recombinant Proteins / chemistry*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Oligonucleotide Probes
  • Recombinant Proteins