[A multistage metrologic approach to the problem of evaluation of the results of the quantitative enzyme immunoassay determination of antitoxic antibodies]

V F Petrov; A M Nikolaeva; V A Ivanova; T S Shobukhova; G M Tumanova

[A multistage metrologic approach to the problem of evaluation of the results of the quantitative enzyme immunoassay determination of antitoxic antibodies]

Zh Mikrobiol Epidemiol Immunobiol. 1998 Mar-Apr:(2):43-6.

[Article in Russian]

Authors

V F Petrov¹, A M Nikolaeva, V A Ivanova, T S Shobukhova, G M Tumanova

Affiliation

¹ Tarasevich State Research Institute for Standardization and Control of Medical Biological Preparations, Moscow, Russia.

PMID: 9662797

Abstract

To evaluate a kit for the enzyme immunoassay (ELISA), the metrological approach was used: the total error of the method for the quantitative determination of antibodies, regarded as a multistage process, was determined as the result of the accumulation of errors made in measurements at different stages. The proposed algorithm made it possible to attest the positive control serum, to determine the total error of measurements, to mark a linear section in a limited range of values on a graduation diagram. This led to obtaining well-grounded results, comparable with the results of the reaction of neutralization at good correlation (lc = 0.9).

Publication types

English Abstract

MeSH terms

Algorithms*
Antibodies, Bacterial / analysis*
Diphtheria Antitoxin / immunology*
Enzyme-Linked Immunosorbent Assay*
Evaluation Studies as Topic
Humans
Linear Models
Reproducibility of Results
Tetanus Antitoxin / immunology*

Substances

Antibodies, Bacterial
Diphtheria Antitoxin
Tetanus Antitoxin