Advanced oxidation protein products as novel mediators of inflammation and monocyte activation in chronic renal failure

J Immunol. 1998 Sep 1;161(5):2524-32.

Abstract

We previously demonstrated the presence of advanced oxidation protein products (AOPP), a novel marker of oxidative stress in the plasma of uremic patients receiving maintenance dialysis. The present study in a cohort of 162 uremic patients showed that plasma concentrations of AOPP increased with progression of chronic renal failure and were closely related to advanced glycation end products (AGE)-pentosidine (r = 0.52, p < 0.001), taken as a marker of AGE. In vivo, the relevance of AOPP and AGE-pentosidine in monocyte-mediated inflammatory syndrome associated with uremia was evidenced by close correlations between AOPP or AGE-pentosidine and monocyte activation markers, including neopterin, IL-1R antagonist, TNF-alpha, and TNF soluble receptors (TNF-sR55 and TNF-sR75). To determine the mechanisms by which AOPP and AGE could be directly involved in monocyte activation, AOPP-human serum albumin (HSA) and AGE-HSA were produced in vitro by treating HSA with oxidants or glucose, respectively. Spectroscopic analysis confirmed that AOPP-HSA contains carbonyls and dityrosine. Both AOPP-HSA and AGE-HSA, but not purified dityrosine, were capable of triggering the oxidative burst of human monocytes in cultures. The AOPP-HSA-induced respiratory burst was dependent on the chlorinated nature of the oxidant and on the molar ratio HSA/HOCI. Collectively, these data first demonstrate that AOPP act as a mediator of oxidative stress and monocyte respiratory burst, which points to monocytes as both target and actor in the immune dysregulation associated with chronic uremia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Biomarkers / blood
  • Cytokines / blood
  • Glycation End Products, Advanced / blood
  • Humans
  • Inflammation / blood
  • Inflammation / immunology
  • Kidney Failure, Chronic / blood
  • Kidney Failure, Chronic / immunology*
  • Kidney Failure, Chronic / pathology*
  • Macrophage Activation* / drug effects
  • Monocytes / immunology*
  • Monocytes / metabolism
  • Oxidative Stress / immunology*
  • Respiratory Burst / drug effects
  • Respiratory Burst / immunology
  • Serum Albumin / physiology

Substances

  • Biomarkers
  • Cytokines
  • Glycation End Products, Advanced
  • Serum Albumin