Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains

J Clin Microbiol. 1998 Nov;36(11):3433-4. doi: 10.1128/JCM.36.11.3433-3434.1998.

Abstract

We compared four tests for antibodies to CagA or VacA, HelicoBlot 2. 0, RIDA Blot Helicobacter, CHIRON RIBA H. pylori SIA, and an enzyme-linked immunosorbent assay using recombinant CagA. Immunoblot assays were accurate for determining Helicobacter pylori status but poor for determining CagA or VacA status (accuracy, 66 to 80% for CagA status and 34 to 67% for VacA status). None can be recommended for determining CagA or VacA status.

Publication types

  • Comparative Study

MeSH terms

  • Antigens, Bacterial* / genetics
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology*
  • Bacterial Toxins / genetics
  • Bacterial Toxins / immunology
  • Bacteriological Techniques
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / statistics & numerical data
  • Evaluation Studies as Topic
  • Helicobacter Infections / diagnosis
  • Helicobacter Infections / epidemiology
  • Helicobacter Infections / microbiology
  • Helicobacter pylori / genetics
  • Helicobacter pylori / immunology*
  • Helicobacter pylori / pathogenicity
  • Humans
  • Immunoblotting / methods*
  • Immunoblotting / statistics & numerical data
  • Sensitivity and Specificity
  • Seroepidemiologic Studies
  • Virulence

Substances

  • Antigens, Bacterial
  • Bacterial Proteins
  • Bacterial Toxins
  • VacA protein, Helicobacter pylori
  • cagA protein, Helicobacter pylori