Abstract
The deoxynucleotide (dNMP) composition of ten strains of C. neoformans was analysed by 32P-labelling and two-dimensional thin-layer chromatography. This technique is very sensitive for detecting rare deoxynucleotide adducts and analogues (minor bases) in DNA. The results indicate considerable variation among strains in DNA nucleotide composition.
MeSH terms
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Autoradiography
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Base Composition
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Brazil
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Chromatography, Thin Layer / methods
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Cryptococcus neoformans / genetics*
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Cryptococcus neoformans / isolation & purification
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DNA, Fungal / chemistry*
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DNA, Fungal / genetics
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DNA, Fungal / isolation & purification
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Deoxyribonucleotides / analysis*
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Genetic Variation*
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Meningitis, Cryptococcal / cerebrospinal fluid
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Meningitis, Cryptococcal / microbiology
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Reproducibility of Results
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Species Specificity
Substances
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DNA, Fungal
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Deoxyribonucleotides