Effects of decidua-conditioned medium and insulin-like growth factor binding protein-1 on trophoblastic matrix metalloproteinases and their inhibitors

Placenta. 1998 Sep;19(7):457-64. doi: 10.1016/s0143-4004(98)91038-4.

Abstract

The regulatory role of in vitro decidualized stromal cells (DESCM) and their main secretory product insulin-like growth factor binding protein-1 (IGFBP-1) was studied on the secretion of trophoblastic gelatinases and tissue inhibitor of metalloproteinase (TIMP-1). First trimester cytotrophoblastic cells (CTB) were obtained from abortions and cultured in vitro in presence or absence of DESCM or IGFBP-1. Secreted gelatinases were analysed in the culture supernatants by zymography and by measurements of the total gelatinolytic activity. TIMP-1, hCG, and fetal fibronectin (fFN) were measured by commercially available immunoassays. DESCM inhibited the total gelatinolytic activity of CTB but increased trophoblastic MMP-9, TIMP-1 and fFN. In contrast, IGFBP-1 increased the total gelatinolytic activity and TIMP-1, had no effect on MMP-2 , MMP-9 or fFN but inhibited hCG. It is concluded that a factor secreted by decidual cells inhibits the gelatinolytic property of trophoblast by increasing TIMP-1. Other decidual factors, as yet unidentified, increase MMP-2 and MMP-9 to an extent which does override the inhibitory effect of TIMP-1. Since in contrast to DESCM, IGFBP-1 increases the total gelatinolytic activity of CTB, it cannot be the primary active decidual factor regulating the proteolytic activity of CTB. The possibility of an integrin-mediated effect of IGFBP-1 on CTB is discussed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Chorionic Gonadotropin / metabolism
  • Collagenases / metabolism*
  • Culture Media, Conditioned / pharmacology
  • Decidua / physiology*
  • Female
  • Fibronectins / metabolism
  • Gelatinases / metabolism*
  • Humans
  • Insulin-Like Growth Factor Binding Protein 1 / pharmacology*
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9
  • Metalloendopeptidases / metabolism*
  • Pregnancy
  • Stromal Cells / drug effects
  • Stromal Cells / enzymology
  • Tissue Inhibitor of Metalloproteinase-1 / metabolism*
  • Trophoblasts / drug effects*
  • Trophoblasts / enzymology

Substances

  • Chorionic Gonadotropin
  • Culture Media, Conditioned
  • Fibronectins
  • Insulin-Like Growth Factor Binding Protein 1
  • Tissue Inhibitor of Metalloproteinase-1
  • Collagenases
  • Gelatinases
  • Metalloendopeptidases
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9