Monoclonal antibody-resistant mutants selected with a respiratory syncytial virus-neutralizing human antibody fab fragment (Fab 19) define a unique epitope on the fusion (F) glycoprotein

Virology. 1998 Dec 20;252(2):373-5. doi: 10.1006/viro.1998.9462.

Abstract

A recombinant human antibody fragment, designated RSV Fab 19, efficiently neutralizes respiratory syncytial virus (RSV). Here we report the results of our sequence analysis of antibody escape mutants that identified F glycoprotein amino acids critical for binding of human or murine RSV F-neutralizing antibodies.

MeSH terms

  • Amino Acid Substitution
  • Animals
  • Antibodies, Monoclonal*
  • Cell Line
  • Epitopes / analysis*
  • Humans
  • Immunoglobulin Fab Fragments*
  • Neutralization Tests
  • Point Mutation
  • Recombinant Proteins
  • Respiratory Syncytial Viruses / genetics
  • Respiratory Syncytial Viruses / immunology*
  • Tumor Cells, Cultured
  • Viral Fusion Proteins / analysis
  • Viral Fusion Proteins / genetics
  • Viral Fusion Proteins / immunology*

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Immunoglobulin Fab Fragments
  • Recombinant Proteins
  • Viral Fusion Proteins