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Characterization of human type III collagen expressed in a baculovirus system. Production of a protein with a stable triple helix requires coexpression with the two types of recombinant prolyl 4-hydroxylase subunit.
Lamberg A, Helaakoski T, Myllyharju J, Peltonen S, Notbohm H, Pihlajaniemi T, Kivirikko KI. Lamberg A, et al. Among authors: myllyharju j. J Biol Chem. 1996 May 17;271(20):11988-95. doi: 10.1074/jbc.271.20.11988. J Biol Chem. 1996. PMID: 8662631 Free article.
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
Annunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI. Annunen P, et al. Among authors: myllyharju j. J Biol Chem. 1997 Jul 11;272(28):17342-8. doi: 10.1074/jbc.272.28.17342. J Biol Chem. 1997. PMID: 9211872 Free article.
Expression of wild-type and modified proalpha chains of human type I procollagen in insect cells leads to the formation of stable [alpha1(I)]2alpha2(I) collagen heterotrimers and [alpha1(I)]3 homotrimers but not [alpha2(I)]3 homotrimers.
Myllyharju J, Lamberg A, Notbohm H, Fietzek PP, Pihlajaniemi T, Kivirikko KI. Myllyharju J, et al. J Biol Chem. 1997 Aug 29;272(35):21824-30. doi: 10.1074/jbc.272.35.21824. J Biol Chem. 1997. PMID: 9268313 Free article.
Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase.
Vuorela A, Myllyharju J, Nissi R, Pihlajaniemi T, Kivirikko KI. Vuorela A, et al. Among authors: myllyharju j. EMBO J. 1997 Nov 17;16(22):6702-12. doi: 10.1093/emboj/16.22.6702. EMBO J. 1997. PMID: 9362485 Free PMC article.
Substrate specificity of human prolyl-4-hydroxylase.
Tandon M, Wu M, Begley TP, Myllyharju J, Pirskanen A, Kivirikko K. Tandon M, et al. Among authors: myllyharju j. Bioorg Med Chem Lett. 1998 May 19;8(10):1139-44. doi: 10.1016/s0960-894x(98)00183-8. Bioorg Med Chem Lett. 1998. PMID: 9871723 Free article.
149 results