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Identification of an ADAMTS-4 cleavage motif using phage display leads to the development of fluorogenic peptide substrates and reveals matrilin-3 as a novel substrate.
Hills R, Mazzarella R, Fok K, Liu M, Nemirovskiy O, Leone J, Zack MD, Arner EC, Viswanathan M, Abujoub A, Muruganandam A, Sexton DJ, Bassill GJ, Sato AK, Malfait AM, Tortorella MD. Hills R, et al. J Biol Chem. 2007 Apr 13;282(15):11101-9. doi: 10.1074/jbc.M611588200. Epub 2007 Feb 20. J Biol Chem. 2007. PMID: 17311924 Free article.
High resolution crystal structure of the catalytic domain of ADAMTS-5 (aggrecanase-2).
Shieh HS, Mathis KJ, Williams JM, Hills RL, Wiese JF, Benson TE, Kiefer JR, Marino MH, Carroll JN, Leone JW, Malfait AM, Arner EC, Tortorella MD, Tomasselli A. Shieh HS, et al. Among authors: leone jw. J Biol Chem. 2008 Jan 18;283(3):1501-1507. doi: 10.1074/jbc.M705879200. Epub 2007 Nov 8. J Biol Chem. 2008. PMID: 17991750 Free article.
Expression, purification, and characterization of TYK-2 kinase domain, a member of the Janus kinase family.
Korniski B, Wittwer AJ, Emmons TL, Hall T, Brown S, Wrightstone AD, Hirsch JL, Gormley JA, Weinberg RA, Leone JW, Day JE, Chrencik JE, Sommers CD, Fischer HD, Tomasselli AG. Korniski B, et al. Among authors: leone jw. Biochem Biophys Res Commun. 2010 May 28;396(2):543-8. doi: 10.1016/j.bbrc.2010.04.141. Epub 2010 May 8. Biochem Biophys Res Commun. 2010. PMID: 20435017
Expression, purification, characterization and crystallization of non- and phosphorylated states of JAK2 and JAK3 kinase domain.
Hall T, Emmons TL, Chrencik JE, Gormley JA, Weinberg RA, Leone JW, Hirsch JL, Saabye MJ, Schindler JF, Day JE, Williams JM, Kiefer JR, Lightle SA, Harris MS, Guru S, Fischer HD, Tomasselli AG. Hall T, et al. Among authors: leone jw. Protein Expr Purif. 2010 Jan;69(1):54-63. doi: 10.1016/j.pep.2009.09.011. Epub 2009 Sep 23. Protein Expr Purif. 2010. PMID: 19781647
Structural and thermodynamic characterization of the TYK2 and JAK3 kinase domains in complex with CP-690550 and CMP-6.
Chrencik JE, Patny A, Leung IK, Korniski B, Emmons TL, Hall T, Weinberg RA, Gormley JA, Williams JM, Day JE, Hirsch JL, Kiefer JR, Leone JW, Fischer HD, Sommers CD, Huang HC, Jacobsen EJ, Tenbrink RE, Tomasselli AG, Benson TE. Chrencik JE, et al. Among authors: leone jw. J Mol Biol. 2010 Jul 16;400(3):413-33. doi: 10.1016/j.jmb.2010.05.020. Epub 2010 May 15. J Mol Biol. 2010. PMID: 20478313
Large-scale purification of human BACE expressed in mammalian cells and removal of the prosegment with HIV-1 protease to improve crystal diffraction.
Emmons TL, Shuck ME, Babcock MS, Holloway JS, Leone JW, Durbin JD, Paddock DJ, Prince DB, Heinrikson RL, Fischer HD, Bienkowski MJ, Benson TE, Tomasselli AG. Emmons TL, et al. Among authors: leone jw. Protein Pept Lett. 2008;15(2):119-30. doi: 10.2174/092986608783489599. Protein Pept Lett. 2008. PMID: 18289104
40 results