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Replacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate.
De Anda R, Lara AR, Hernández V, Hernández-Montalvo V, Gosset G, Bolívar F, Ramírez OT. De Anda R, et al. Among authors: ramirez ot. Metab Eng. 2006 May;8(3):281-90. doi: 10.1016/j.ymben.2006.01.002. Epub 2006 Mar 6. Metab Eng. 2006. PMID: 16517196
Growth recovery on glucose under aerobic conditions of an Escherichia coli strain carrying a phosphoenolpyruvate:carbohydrate phosphotransferase system deletion by inactivating arcA and overexpressing the genes coding for glucokinase and galactose permease.
Flores N, Leal L, Sigala JC, de Anda R, Escalante A, Martínez A, Ramírez OT, Gosset G, Bolivar F. Flores N, et al. Among authors: ramirez ot. J Mol Microbiol Biotechnol. 2007;13(1-3):105-16. doi: 10.1159/000103602. J Mol Microbiol Biotechnol. 2007. PMID: 17693718
96 results