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The metabolite α-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR.
Chin RM, Fu X, Pai MY, Vergnes L, Hwang H, Deng G, Diep S, Lomenick B, Meli VS, Monsalve GC, Hu E, Whelan SA, Wang JX, Jung G, Solis GM, Fazlollahi F, Kaweeteerawat C, Quach A, Nili M, Krall AS, Godwin HA, Chang HR, Faull KF, Guo F, Jiang M, Trauger SA, Saghatelian A, Braas D, Christofk HR, Clarke CF, Teitell MA, Petrascheck M, Reue K, Jung ME, Frand AR, Huang J. Chin RM, et al. Among authors: huang j. Nature. 2014 Jun 19;510(7505):397-401. doi: 10.1038/nature13264. Epub 2014 May 14. Nature. 2014. PMID: 24828042 Free PMC article.
Target identification using drug affinity responsive target stability (DARTS).
Lomenick B, Hao R, Jonai N, Chin RM, Aghajan M, Warburton S, Wang J, Wu RP, Gomez F, Loo JA, Wohlschlegel JA, Vondriska TM, Pelletier J, Herschman HR, Clardy J, Clarke CF, Huang J. Lomenick B, et al. Among authors: huang j. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21984-9. doi: 10.1073/pnas.0910040106. Epub 2009 Dec 7. Proc Natl Acad Sci U S A. 2009. PMID: 19995983 Free PMC article.
Chemical genetics screen for enhancers of rapamycin identifies a specific inhibitor of an SCF family E3 ubiquitin ligase.
Aghajan M, Jonai N, Flick K, Fu F, Luo M, Cai X, Ouni I, Pierce N, Tang X, Lomenick B, Damoiseaux R, Hao R, Del Moral PM, Verma R, Li Y, Li C, Houk KN, Jung ME, Zheng N, Huang L, Deshaies RJ, Kaiser P, Huang J. Aghajan M, et al. Among authors: huang l, huang j. Nat Biotechnol. 2010 Jul;28(7):738-42. doi: 10.1038/nbt.1645. Epub 2010 Jun 27. Nat Biotechnol. 2010. PMID: 20581845 Free PMC article.
Identification of direct protein targets of small molecules.
Lomenick B, Olsen RW, Huang J. Lomenick B, et al. Among authors: huang j. ACS Chem Biol. 2011 Jan 21;6(1):34-46. doi: 10.1021/cb100294v. Epub 2010 Nov 30. ACS Chem Biol. 2011. PMID: 21077692 Free PMC article. Review.
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