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A procedure for setting up high-throughput nanolitre crystallization experiments. Crystallization workflow for initial screening, automated storage, imaging and optimization.
Walter TS, Diprose JM, Mayo CJ, Siebold C, Pickford MG, Carter L, Sutton GC, Berrow NS, Brown J, Berry IM, Stewart-Jones GB, Grimes JM, Stammers DK, Esnouf RM, Jones EY, Owens RJ, Stuart DI, Harlos K. Walter TS, et al. Among authors: grimes jm. Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):651-7. doi: 10.1107/S0907444905007808. Epub 2005 May 26. Acta Crystallogr D Biol Crystallogr. 2005. PMID: 15930615 Free PMC article.
Semi-automated microseeding of nanolitre crystallization experiments.
Walter TS, Mancini EJ, Kadlec J, Graham SC, Assenberg R, Ren J, Sainsbury S, Owens RJ, Stuart DI, Grimes JM, Harlos K. Walter TS, et al. Among authors: grimes jm. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Jan 1;64(Pt 1):14-8. doi: 10.1107/S1744309107057260. Epub 2007 Dec 20. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008. PMID: 18097093 Free PMC article.
In situ macromolecular crystallography using microbeams.
Axford D, Owen RL, Aishima J, Foadi J, Morgan AW, Robinson JI, Nettleship JE, Owens RJ, Moraes I, Fry EE, Grimes JM, Harlos K, Kotecha A, Ren J, Sutton G, Walter TS, Stuart DI, Evans G. Axford D, et al. Among authors: grimes jm. Acta Crystallogr D Biol Crystallogr. 2012 May;68(Pt 5):592-600. doi: 10.1107/S0907444912006749. Epub 2012 Apr 17. Acta Crystallogr D Biol Crystallogr. 2012. PMID: 22525757 Free PMC article.
Expression, purification and crystallization of the ectodomain of the envelope glycoprotein E2 from Bovine viral diarrhoea virus.
Iourin O, Harlos K, El Omari K, Lu W, Kadlec J, Iqbal M, Meier C, Palmer A, Jones I, Thomas C, Brownlie J, Grimes JM, Stuart DI. Iourin O, et al. Among authors: grimes jm. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jan 1;69(Pt 1):35-8. doi: 10.1107/S1744309112049184. Epub 2012 Dec 15. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013. PMID: 23295482 Free PMC article.
197 results