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Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.
Aceti DJ, Bingman CA, Wrobel RL, Frederick RO, Makino S, Nichols KW, Sahu SC, Bergeman LF, Blommel PG, Cornilescu CC, Gromek KA, Seder KD, Hwang S, Primm JG, Sabat G, Vojtik FC, Volkman BF, Zolnai Z, Phillips GN Jr, Markley JL, Fox BG. Aceti DJ, et al. Among authors: volkman bf. J Struct Funct Genomics. 2015 Jun;16(2):67-80. doi: 10.1007/s10969-015-9198-1. Epub 2015 Apr 9. J Struct Funct Genomics. 2015. PMID: 25854603 Free PMC article.
Microscopic pKa values of Escherichia coli thioredoxin.
Chivers PT, Prehoda KE, Volkman BF, Kim BM, Markley JL, Raines RT. Chivers PT, et al. Among authors: volkman bf. Biochemistry. 1997 Dec 2;36(48):14985-91. doi: 10.1021/bi970071j. Biochemistry. 1997. PMID: 9398223
230 results