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Carbamylation reduces the capacity of IgG for hexamerization and complement activation.
Lubbers R, Oostindie SC, Dijkstra DJ, Parren PWHI, Verheul MK, Abendstein L, Sharp TH, de Ru A, Janssen GMC, van Veelen PA, van den Bremer ETJ, Bleijlevens B, de Kreuk BJ, Beurskens FJ, Trouw LA. Lubbers R, et al. Among authors: van den bremer etj. Clin Exp Immunol. 2020 Apr;200(1):1-11. doi: 10.1111/cei.13411. Epub 2020 Jan 5. Clin Exp Immunol. 2020. PMID: 31853959 Free PMC article.
In-depth qualitative and quantitative analysis of composite glycosylation profiles and other micro-heterogeneity on intact monoclonal antibodies by high-resolution native mass spectrometry using a modified Orbitrap.
Rosati S, van den Bremer ET, Schuurman J, Parren PW, Kamerling JP, Heck AJ. Rosati S, et al. Among authors: van den bremer et. MAbs. 2013 Nov-Dec;5(6):917-24. doi: 10.4161/mabs.26282. Epub 2013 Aug 28. MAbs. 2013. PMID: 23995615 Free PMC article.
Production of stable bispecific IgG1 by controlled Fab-arm exchange: scalability from bench to large-scale manufacturing by application of standard approaches.
Gramer MJ, van den Bremer ET, van Kampen MD, Kundu A, Kopfmann P, Etter E, Stinehelfer D, Long J, Lannom T, Noordergraaf EH, Gerritsen J, Labrijn AF, Schuurman J, van Berkel PH, Parren PW. Gramer MJ, et al. Among authors: van den bremer et. MAbs. 2013 Nov-Dec;5(6):962-73. doi: 10.4161/mabs.26233. Epub 2013 Aug 22. MAbs. 2013. PMID: 23995617 Free PMC article.
Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange.
Labrijn AF, Meesters JI, de Goeij BE, van den Bremer ET, Neijssen J, van Kampen MD, Strumane K, Verploegen S, Kundu A, Gramer MJ, van Berkel PH, van de Winkel JG, Schuurman J, Parren PW. Labrijn AF, et al. Among authors: van den bremer et, van kampen md, van berkel ph, van de winkel jg. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5145-50. doi: 10.1073/pnas.1220145110. Epub 2013 Mar 11. Proc Natl Acad Sci U S A. 2013. PMID: 23479652 Free PMC article.
Controlled Fab-arm exchange for the generation of stable bispecific IgG1.
Labrijn AF, Meesters JI, Priem P, de Jong RN, van den Bremer ET, van Kampen MD, Gerritsen AF, Schuurman J, Parren PW. Labrijn AF, et al. Among authors: van den bremer et. Nat Protoc. 2014 Oct;9(10):2450-63. doi: 10.1038/nprot.2014.169. Epub 2014 Sep 25. Nat Protoc. 2014. PMID: 25255089
Biophysical Characterization and Stability of Modified IgG1 Antibodies with Different Hexamerization Propensities.
van Kampen MD, Kuipers-De Wilt LHAM, van Egmond ML, Reinders-Blankert P, van den Bremer ETJ, Wang G, Heck AJR, Parren PWHI, Beurskens FJ, Schuurman J, de Jong RN. van Kampen MD, et al. Among authors: van den bremer etj. J Pharm Sci. 2022 Jun;111(6):1587-1598. doi: 10.1016/j.xphs.2022.02.016. Epub 2022 Feb 27. J Pharm Sci. 2022. PMID: 35235843 Free article.
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