Conklin BR - Search Results

1

Using Digital Polymerase Chain Reaction to Detect Single-Nucleotide Substitutions Induced by Genome Editing.

Miyaoka Y, Chan AH, Conklin BR. Miyaoka Y, et al. Among authors: conklin br. Cold Spring Harb Protoc. 2016 Aug 1;2016(8). doi: 10.1101/pdb.prot086801. Cold Spring Harb Protoc. 2016. PMID: 27250210

2

Isolation of single-base genome-edited human iPS cells without antibiotic selection.

Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR. Miyaoka Y, et al. Among authors: conklin br. Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 2014 Feb 9. Nat Methods. 2014. PMID: 24509632 Free PMC article.

3

CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs.

Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR. Mandegar MA, et al. Among authors: conklin br. Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 10.1016/j.stem.2016.01.022. Epub 2016 Mar 10. Cell Stem Cell. 2016. PMID: 26971820 Free PMC article.

4

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing.

Miyaoka Y, Berman JR, Cooper SB, Mayerl SJ, Chan AH, Zhang B, Karlin-Neumann GA, Conklin BR. Miyaoka Y, et al. Among authors: conklin br. Sci Rep. 2016 Mar 31;6:23549. doi: 10.1038/srep23549. Sci Rep. 2016. PMID: 27030102 Free PMC article.

5

Detecting Single-Nucleotide Substitutions Induced by Genome Editing.

Miyaoka Y, Chan AH, Conklin BR. Miyaoka Y, et al. Among authors: conklin br. Cold Spring Harb Protoc. 2016 Aug 1;2016(8). doi: 10.1101/pdb.top090845. Cold Spring Harb Protoc. 2016. PMID: 27250209

6

Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity.

Nguyen DP, Miyaoka Y, Gilbert LA, Mayerl SJ, Lee BH, Weissman JS, Conklin BR, Wells JA. Nguyen DP, et al. Among authors: conklin br. Nat Commun. 2016 Jul 1;7:12009. doi: 10.1038/ncomms12009. Nat Commun. 2016. PMID: 27363581 Free PMC article.

7

Engineered human pluripotent-stem-cell-derived intestinal tissues with a functional enteric nervous system.

Workman MJ, Mahe MM, Trisno S, Poling HM, Watson CL, Sundaram N, Chang CF, Schiesser J, Aubert P, Stanley EG, Elefanty AG, Miyaoka Y, Mandegar MA, Conklin BR, Neunlist M, Brugmann SA, Helmrath MA, Wells JM. Workman MJ, et al. Among authors: conklin br. Nat Med. 2017 Jan;23(1):49-59. doi: 10.1038/nm.4233. Epub 2016 Nov 21. Nat Med. 2017. PMID: 27869805 Free PMC article.

8

Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci Using Droplet Digital PCR.

Miyaoka Y, Mayerl SJ, Chan AH, Conklin BR. Miyaoka Y, et al. Among authors: conklin br. Methods Mol Biol. 2018;1768:349-362. doi: 10.1007/978-1-4939-7778-9_20. Methods Mol Biol. 2018. PMID: 29717453 Free PMC article.

9

CRISPR off-target detection with DISCOVER-seq.

Wienert B, Wyman SK, Yeh CD, Conklin BR, Corn JE. Wienert B, et al. Among authors: conklin br. Nat Protoc. 2020 May;15(5):1775-1799. doi: 10.1038/s41596-020-0309-5. Epub 2020 Apr 20. Nat Protoc. 2020. PMID: 32313254 Free PMC article.

10

Rapid, precise quantification of large DNA excisions and inversions by ddPCR.

Watry HL, Feliciano CM, Gjoni K, Takahashi G, Miyaoka Y, Conklin BR, Judge LM. Watry HL, et al. Among authors: conklin br. Sci Rep. 2020 Sep 10;10(1):14896. doi: 10.1038/s41598-020-71742-z. Sci Rep. 2020. PMID: 32913194 Free PMC article.

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