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Protocol to Design, Clone, and Validate sgRNAs for In Vivo Reverse Genetic Studies.
Hunker AC, Zweifel LS. Hunker AC, et al. STAR Protoc. 2020 Sep 18;1(2):100070. doi: 10.1016/j.xpro.2020.100070. Epub 2020 Jul 22. STAR Protoc. 2020. PMID: 33043306 Free PMC article.
Current methods determining In Vivo on-target mutagenesis have been limited by the ability to isolate virally transduced cells. This protocol optimizes a workflow for the design, cloning, and validation of sgRNAs delivered by AAVs In Vivo that can be applied to any target …
Current methods determining In Vivo on-target mutagenesis have been limited by the ability to isolate virally transduced cells. This protoco …
A midbrain dynorphin circuit promotes threat generalization.
Fellinger L, Jo YS, Hunker AC, Soden ME, Elum J, Juarez B, Zweifel LS. Fellinger L, et al. Curr Biol. 2021 Oct 11;31(19):4388-4396.e5. doi: 10.1016/j.cub.2021.07.047. Epub 2021 Aug 12. Curr Biol. 2021. PMID: 34388372 Free PMC article.
22 results