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Engineered CRISPR-Cas9 nuclease with expanded targeting space.
Nishimasu H, Shi X, Ishiguro S, Gao L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki M, Hirano H, Aburatani H, Ishitani R, Ikawa M, Yachie N, Zhang F, Nureki O. Nishimasu H, et al. Among authors: okazaki s. Science. 2018 Sep 21;361(6408):1259-1262. doi: 10.1126/science.aas9129. Epub 2018 Aug 30. Science. 2018. PMID: 30166441 Free PMC article.
Structure of the miniature type V-F CRISPR-Cas effector enzyme.
Takeda SN, Nakagawa R, Okazaki S, Hirano H, Kobayashi K, Kusakizako T, Nishizawa T, Yamashita K, Nishimasu H, Nureki O. Takeda SN, et al. Among authors: okazaki s. Mol Cell. 2021 Feb 4;81(3):558-570.e3. doi: 10.1016/j.molcel.2020.11.035. Epub 2020 Dec 16. Mol Cell. 2021. PMID: 33333018 Free article.
Amplification-free RNA detection with CRISPR-Cas13.
Shinoda H, Taguchi Y, Nakagawa R, Makino A, Okazaki S, Nakano M, Muramoto Y, Takahashi C, Takahashi I, Ando J, Noda T, Nureki O, Nishimasu H, Watanabe R. Shinoda H, et al. Among authors: okazaki s. Commun Biol. 2021 Apr 19;4(1):476. doi: 10.1038/s42003-021-02001-8. Commun Biol. 2021. PMID: 33875803 Free PMC article.
Structure of the type V-C CRISPR-Cas effector enzyme.
Kurihara N, Nakagawa R, Hirano H, Okazaki S, Tomita A, Kobayashi K, Kusakizako T, Nishizawa T, Yamashita K, Scott DA, Nishimasu H, Nureki O. Kurihara N, et al. Among authors: okazaki s. Mol Cell. 2022 May 19;82(10):1865-1877.e4. doi: 10.1016/j.molcel.2022.03.006. Epub 2022 Apr 1. Mol Cell. 2022. PMID: 35366394 Free PMC article.
Structure of the IscB-ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9.
Kato K, Okazaki S, Kannan S, Altae-Tran H, Esra Demircioglu F, Isayama Y, Ishikawa J, Fukuda M, Macrae RK, Nishizawa T, Makarova KS, Koonin EV, Zhang F, Nishimasu H. Kato K, et al. Among authors: okazaki s. Nat Commun. 2022 Nov 7;13(1):6719. doi: 10.1038/s41467-022-34378-3. Nat Commun. 2022. PMID: 36344504 Free PMC article.
Structural mechanism of bridge RNA-guided recombination.
Hiraizumi M, Perry NT, Durrant MG, Soma T, Nagahata N, Okazaki S, Athukoralage JS, Isayama Y, Pai JJ, Pawluk A, Konermann S, Yamashita K, Hsu PD, Nishimasu H. Hiraizumi M, et al. Among authors: okazaki s. Nature. 2024 Jun;630(8018):994-1002. doi: 10.1038/s41586-024-07570-2. Epub 2024 Jun 26. Nature. 2024. PMID: 38926616 Free PMC article.
Crystal structure of heliorhodopsin.
Shihoya W, Inoue K, Singh M, Konno M, Hososhima S, Yamashita K, Ikeda K, Higuchi A, Izume T, Okazaki S, Hashimoto M, Mizutori R, Tomida S, Yamauchi Y, Abe-Yoshizumi R, Katayama K, Tsunoda SP, Shibata M, Furutani Y, Pushkarev A, Béjà O, Uchihashi T, Kandori H, Nureki O. Shihoya W, et al. Among authors: okazaki s. Nature. 2019 Oct;574(7776):132-136. doi: 10.1038/s41586-019-1604-6. Epub 2019 Sep 25. Nature. 2019. PMID: 31554965
1,303 results