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Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins.
Mangeot PE, Risson V, Fusil F, Marnef A, Laurent E, Blin J, Mournetas V, Massouridès E, Sohier TJM, Corbin A, Aubé F, Teixeira M, Pinset C, Schaeffer L, Legube G, Cosset FL, Verhoeyen E, Ohlmann T, Ricci EP. Mangeot PE, et al. Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z. Nat Commun. 2019. PMID: 30604748 Free PMC article.
Viruses traverse the human proteome through peptide interfaces that can be biomimetically leveraged for drug discovery.
Meyniel-Schicklin L, Amaudrut J, Mallinjoud P, Guillier F, Mangeot PE, Lines L, Aublin-Gex A, Scholtes C, Punginelli C, Joly S, Vasseur F, Manet E, Gruffat H, Henry T, Halitim F, Paparin JL, Machin P, Darteil R, Sampson D, Mikaelian I, Lane L, Navratil V, Golinelli-Cohen MP, Terzi F, André P, Lotteau V, Vonderscher J, Meldrum EC, de Chassey B. Meyniel-Schicklin L, et al. Among authors: mangeot pe. Proc Natl Acad Sci U S A. 2024 Jan 30;121(5):e2308776121. doi: 10.1073/pnas.2308776121. Epub 2024 Jan 22. Proc Natl Acad Sci U S A. 2024. PMID: 38252831 Free PMC article.
Nanoblades allow high-level genome editing in murine and human organoids.
Tiroille V, Krug A, Bokobza E, Kahi M, Bulcaen M, Ensinck MM, Geurts MH, Hendriks D, Vermeulen F, Larbret F, Gutierrez-Guerrero A, Chen Y, Van Zundert I, Rocha S, Rios AC, Medaer L, Gijsbers R, Mangeot PE, Clevers H, Carlon MS, Bost F, Verhoeyen E. Tiroille V, et al. Among authors: mangeot pe. Mol Ther Nucleic Acids. 2023 Jun 8;33:57-74. doi: 10.1016/j.omtn.2023.06.004. eCollection 2023 Sep 12. Mol Ther Nucleic Acids. 2023. PMID: 37435135 Free PMC article.
Baboon Envelope Pseudotyped "Nanoblades" Carrying Cas9/gRNA Complexes Allow Efficient Genome Editing in Human T, B, and CD34+ Cells and Knock-in of AAV6-Encoded Donor DNA in CD34+ Cells.
Gutierrez-Guerrero A, Abrey Recalde MJ, Mangeot PE, Costa C, Bernadin O, Périan S, Fusil F, Froment G, Martinez-Turtos A, Krug A, Martin F, Benabdellah K, Ricci EP, Giovannozzi S, Gijsbers R, Ayuso E, Cosset FL, Verhoeyen E. Gutierrez-Guerrero A, et al. Among authors: mangeot pe. Front Genome Ed. 2021 Feb 9;3:604371. doi: 10.3389/fgeed.2021.604371. eCollection 2021. Front Genome Ed. 2021. PMID: 34713246 Free PMC article.
Loop extrusion as a mechanism for formation of DNA damage repair foci.
Arnould C, Rocher V, Finoux AL, Clouaire T, Li K, Zhou F, Caron P, Mangeot PE, Ricci EP, Mourad R, Haber JE, Noordermeer D, Legube G. Arnould C, et al. Among authors: mangeot pe. Nature. 2021 Feb;590(7847):660-665. doi: 10.1038/s41586-021-03193-z. Epub 2021 Feb 17. Nature. 2021. PMID: 33597753 Free PMC article.
27 results