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TaqMan-quantitative PCR assays applied in Neospora caninum knock-outs generated through CRISPR-Cas9 allow to determine the copy numbers of integrated dihydrofolate reductase-thymidylate synthase drug selectable markers.
Rico-San Román L, Hänggeli KPA, Hemphill A, Horcajo P, Collantes-Fernández E, Ortega-Mora LM, Boubaker G. Rico-San Román L, et al. Front Cell Infect Microbiol. 2024 Jun 21;14:1419209. doi: 10.3389/fcimb.2024.1419209. eCollection 2024. Front Cell Infect Microbiol. 2024. PMID: 38975328 Free PMC article.