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A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes.
Bulmer GS, Mattey AP, Parmeggiani F, Williams R, Ledru H, Marchesi A, Seibt LS, Both P, Huang K, Galan MC, Flitsch SL, Green AP, van Munster JM. Bulmer GS, et al. Among authors: seibt ls. Org Biomol Chem. 2021 Jun 30;19(25):5529-5533. doi: 10.1039/d1ob00971k. Org Biomol Chem. 2021. PMID: 34105582 Free PMC article.
Mapping the Initial Stages of a Protective Pathway that Enhances Catalytic Turnover by a Lytic Polysaccharide Monooxygenase.
Zhao J, Zhuo Y, Diaz DE, Shanmugam M, Telfer AJ, Lindley PJ, Kracher D, Hayashi T, Seibt LS, Hardy FJ, Manners O, Hedison TM, Hollywood KA, Spiess R, Cain KM, Diaz-Moreno S, Scrutton NS, Tovborg M, Walton PH, Heyes DJ, Green AP. Zhao J, et al. Among authors: seibt ls. J Am Chem Soc. 2023 Sep 20;145(37):20672-20682. doi: 10.1021/jacs.3c06607. Epub 2023 Sep 9. J Am Chem Soc. 2023. PMID: 37688545 Free PMC article.
Development of a colourimetric assay for glycosynthases.
Hayes MR, Bochinsky KA, Seibt LS, Elling L, Pietruszka J. Hayes MR, et al. Among authors: seibt ls. J Biotechnol. 2017 Sep 10;257:162-170. doi: 10.1016/j.jbiotec.2017.02.005. Epub 2017 Feb 11. J Biotechnol. 2017. PMID: 28193496