Using promoter replacement and selection for loss of heterozygosity to generate an industrially applicable sake yeast strain that homozygously overproduces isoamyl acetate.
Sahara H, Kotaka A, Kondo A, Ueda M, Hata Y.
Sahara H, et al. Among authors: kotaka a.
J Biosci Bioeng. 2009 Nov;108(5):359-64. doi: 10.1016/j.jbiosc.2009.05.002.
J Biosci Bioeng. 2009.
PMID: 19804856
By application of the high-efficiency loss of heterozygosity (HELOH) method for disrupting genes in diploid sake yeast (Kotaka et al., Appl. Microbiol. Biotechnol., 82, 387-395 (2009)), we constructed, from a heterozygous integrant, a homozygous diploid that …
By application of the high-efficiency loss of heterozygosity (HELOH) method for disrupting genes in diploid sake yeast (Kotaka et al. …