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Uncovering a membrane-distal conformation of KRAS available to recruit RAF to the plasma membrane.
Van QN, López CA, Tonelli M, Taylor T, Niu B, Stanley CB, Bhowmik D, Tran TH, Frank PH, Messing S, Alexander P, Scott D, Ye X, Drew M, Chertov O, Lösche M, Ramanathan A, Gross ML, Hengartner NW, Westler WM, Markley JL, Simanshu DK, Nissley DV, Gillette WK, Esposito D, McCormick F, Gnanakaran S, Heinrich F, Stephen AG. Van QN, et al. Among authors: messing s. Proc Natl Acad Sci U S A. 2020 Sep 29;117(39):24258-24268. doi: 10.1073/pnas.2006504117. Epub 2020 Sep 10. Proc Natl Acad Sci U S A. 2020. PMID: 32913056 Free PMC article.
Quantitative biophysical analysis defines key components modulating recruitment of the GTPase KRAS to the plasma membrane.
Lakshman B, Messing S, Schmid EM, Clogston JD, Gillette WK, Esposito D, Kessing B, Fletcher DA, Nissley DV, McCormick F, Stephen AG, Jean-Francois FL. Lakshman B, et al. Among authors: messing s. J Biol Chem. 2019 Feb 8;294(6):2193-2207. doi: 10.1074/jbc.RA118.005669. Epub 2018 Dec 17. J Biol Chem. 2019. PMID: 30559287 Free PMC article.
Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer.
Sherekar M, Han SW, Ghirlando R, Messing S, Drew M, Rabara D, Waybright T, Juneja P, O'Neill H, Stanley CB, Bhowmik D, Ramanathan A, Subramaniam S, Nissley DV, Gillette W, McCormick F, Esposito D. Sherekar M, et al. Among authors: messing s. J Biol Chem. 2020 Jan 24;295(4):1105-1119. doi: 10.1074/jbc.RA119.010934. Epub 2019 Dec 13. J Biol Chem. 2020. PMID: 31836666 Free PMC article.
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays.
Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W. Esposito D, et al. Among authors: messing s. Protein Expr Purif. 2020 Oct;174:105686. doi: 10.1016/j.pep.2020.105686. Epub 2020 Jun 4. Protein Expr Purif. 2020. PMID: 32504802 Free PMC article.
Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays.
Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W. Esposito D, et al. Among authors: messing s. bioRxiv [Preprint]. 2020 May 28:2020.05.27.120204. doi: 10.1101/2020.05.27.120204. bioRxiv. 2020. Update in: Protein Expr Purif. 2020 Oct;174:105686. doi: 10.1016/j.pep.2020.105686 PMID: 32511418 Free PMC article. Updated. Preprint.
Standardization of enzyme-linked immunosorbent assays for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling.
Klumpp-Thomas C, Kalish H, Drew M, Hunsberger S, Snead K, Fay MP, Mehalko J, Shunmugavel A, Wall V, Frank P, Denson JP, Hong M, Gulten G, Messing S, Hicks J, Michael S, Gillette W, Hall MD, Memoli M, Esposito D, Sadtler K. Klumpp-Thomas C, et al. Among authors: messing s. medRxiv [Preprint]. 2020 May 25:2020.05.21.20109280. doi: 10.1101/2020.05.21.20109280. medRxiv. 2020. Update in: Nat Commun. 2021 Jan 4;12(1):113. doi: 10.1038/s41467-020-20383-x PMID: 32511472 Free PMC article. Updated. Preprint.
161 results