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Eukaryotic RNA-guided endonucleases evolved from a unique clade of bacterial enzymes.
Yoon PH, Skopintsev P, Shi H, Chen L, Adler BA, Al-Shimary M, Craig RJ, Loi KJ, DeTurk EC, Li Z, Amerasekera J, Trinidad M, Nisonoff H, Chen K, Lahiri A, Boger R, Jacobsen S, Banfield JF, Doudna JA. Yoon PH, et al. Among authors: jacobsen s. Nucleic Acids Res. 2023 Dec 11;51(22):12414-12427. doi: 10.1093/nar/gkad1053. Nucleic Acids Res. 2023. PMID: 37971304 Free PMC article.
CRISPR-CasΦ from huge phages is a hypercompact genome editor.
Pausch P, Al-Shayeb B, Bisom-Rapp E, Tsuchida CA, Li Z, Cress BF, Knott GJ, Jacobsen SE, Banfield JF, Doudna JA. Pausch P, et al. Science. 2020 Jul 17;369(6501):333-337. doi: 10.1126/science.abb1400. Science. 2020. PMID: 32675376 Free PMC article.
Diverse virus-encoded CRISPR-Cas systems include streamlined genome editors.
Al-Shayeb B, Skopintsev P, Soczek KM, Stahl EC, Li Z, Groover E, Smock D, Eggers AR, Pausch P, Cress BF, Huang CJ, Staskawicz B, Savage DF, Jacobsen SE, Banfield JF, Doudna JA. Al-Shayeb B, et al. Cell. 2022 Nov 23;185(24):4574-4586.e16. doi: 10.1016/j.cell.2022.10.020. Cell. 2022. PMID: 36423580 Free article.
Genome editing in plants using the compact editor CasΦ.
Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna JA, Jacobsen SE. Li Z, et al. Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 10.1073/pnas.2216822120. Epub 2023 Jan 18. Proc Natl Acad Sci U S A. 2023. PMID: 36652483 Free PMC article.
The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation.
Li Z, Wang M, Zhong Z, Gallego-Bartolomé J, Feng S, Jami-Alahmadi Y, Wang X, Wohlschlegel J, Bischof S, Long JA, Jacobsen SE. Li Z, et al. Among authors: jacobsen se. bioRxiv [Preprint]. 2023 Jan 10:2023.01.10.523455. doi: 10.1101/2023.01.10.523455. bioRxiv. 2023. Update in: Nat Commun. 2023 Jul 12;14(1):4135. doi: 10.1038/s41467-023-39751-4. PMID: 36711532 Free PMC article. Updated. Preprint.
The role of MORC3 in silencing transposable elements in mouse embryonic stem cells.
Desai VP, Chouaref J, Wu H, Pastor WA, Kan RL, Oey HM, Li Z, Ho J, Vonk KKD, San Leon Granado D, Christopher MA, Clark AT, Jacobsen SE, Daxinger L. Desai VP, et al. Epigenetics Chromatin. 2021 Oct 27;14(1):49. doi: 10.1186/s13072-021-00420-9. Epigenetics Chromatin. 2021. PMID: 34706774 Free PMC article.
A gene silencing screen uncovers diverse tools for targeted gene repression in Arabidopsis.
Wang M, Zhong Z, Gallego-Bartolomé J, Li Z, Feng S, Kuo HY, Kan RL, Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, Wohlschlegel J, Jacobsen SE. Wang M, et al. Among authors: jacobsen se. Nat Plants. 2023 Mar;9(3):460-472. doi: 10.1038/s41477-023-01362-8. Epub 2023 Mar 6. Nat Plants. 2023. PMID: 36879017 Free PMC article.
2,146 results