IL2RG-deficient minipigs generated via CRISPR/Cas9 technology support the growth of human melanoma-derived tumours

Cell Prolif. 2020 Oct;53(10):e12863. doi: 10.1111/cpr.12863. Epub 2020 Sep 1.

Abstract

Objectives: Immunodeficient mice injected with human cancer cell lines have been used for human oncology studies and anti-cancer drug trials for several decades. However, rodents are not ideal species for modelling human cancer because rodents are physiologically dissimilar to humans. Therefore, anti-tumour drugs tested effective in rodents have a failure rate of 90% or higher in phase III clinical trials. Pigs are similar to humans in size, anatomy, physiology and drug metabolism rate, rendering them a desirable pre-clinical animal model for assessing anti-cancer drugs. However, xenogeneic immune rejection is a major barrier to the use of pigs as hosts for human tumours. Interleukin (IL)-2 receptor γ (IL2RG), a common signalling subunit for multiple immune cytokines including IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21, is required for proper lymphoid development.

Materials and methods: IL2RG-/Y pigs were generated by CRISPR/Cas9 technology, and examined for immunodeficiency and ability to support human oncogenesis.

Results: Compared to age-matched wild-type pigs, IL2RG-/Y pigs exhibited a severely impaired immune system as shown by lymphopenia, lymphoid organ atrophy, poor immunoglobulin function, and T- and NK-cell deficiency. Human melanoma Mel888 cells generated tumours in IL2RG-/Y pigs but not in wild-type littermates. The human tumours grew faster in IL2RG-/Y pigs than in nude mice.

Conclusions: Our results indicate that these pigs are promising hosts for modelling human cancer in vivo, which may aid in the discovery and development of anti-cancer drugs.

Keywords: IL2RG knockout minipig; anti-cancer drug trials; in vivo tumour model; tumours; xenogeneic immune rejection.

MeSH terms

  • Animals
  • Animals, Genetically Modified / metabolism
  • CRISPR-Cas Systems / genetics*
  • Cell Line, Tumor
  • Disease Models, Animal
  • Gene Editing
  • Humans
  • Immune System / metabolism
  • Interleukin Receptor Common gamma Subunit / antagonists & inhibitors
  • Interleukin Receptor Common gamma Subunit / genetics
  • Interleukin Receptor Common gamma Subunit / metabolism*
  • Lymphopenia / pathology
  • Melanoma / metabolism
  • Melanoma / pathology
  • RNA Interference
  • RNA, Small Interfering / metabolism
  • Skin Neoplasms / metabolism
  • Skin Neoplasms / mortality
  • Skin Neoplasms / pathology*
  • Survival Rate
  • Swine
  • Swine, Miniature
  • Transplantation, Heterologous

Substances

  • Interleukin Receptor Common gamma Subunit
  • RNA, Small Interfering