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High-throughput functional genomics using CRISPR-Cas9.
Shalem O, Sanjana NE, Zhang F. Shalem O, et al. Nat Rev Genet. 2015 May;16(5):299-311. doi: 10.1038/nrg3899. Epub 2015 Apr 9. Nat Rev Genet. 2015. PMID: 25854182 Free PMC article. Review.
Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis.
Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA. Chen S, et al. Cell. 2015 Mar 12;160(6):1246-60. doi: 10.1016/j.cell.2015.02.038. Epub 2015 Mar 5. Cell. 2015. PMID: 25748654 Free PMC article.
BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis.
Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE. Canver MC, et al. Nature. 2015 Nov 12;527(7577):192-7. doi: 10.1038/nature15521. Epub 2015 Sep 16. Nature. 2015. PMID: 26375006 Free PMC article.
Hypoxia as a therapy for mitochondrial disease.
Jain IH, Zazzeron L, Goli R, Alexa K, Schatzman-Bone S, Dhillon H, Goldberger O, Peng J, Shalem O, Sanjana NE, Zhang F, Goessling W, Zapol WM, Mootha VK. Jain IH, et al. Science. 2016 Apr 1;352(6281):54-61. doi: 10.1126/science.aad9642. Epub 2016 Feb 25. Science. 2016. PMID: 26917594 Free PMC article.
A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks.
Parnas O, Jovanovic M, Eisenhaure TM, Herbst RH, Dixit A, Ye CJ, Przybylski D, Platt RJ, Tirosh I, Sanjana NE, Shalem O, Satija R, Raychowdhury R, Mertins P, Carr SA, Zhang F, Hacohen N, Regev A. Parnas O, et al. Cell. 2015 Jul 30;162(3):675-86. doi: 10.1016/j.cell.2015.06.059. Epub 2015 Jul 16. Cell. 2015. PMID: 26189680 Free PMC article.
Genome-scale CRISPR pooled screens.
Sanjana NE. Sanjana NE. Anal Biochem. 2017 Sep 1;532:95-99. doi: 10.1016/j.ab.2016.05.014. Epub 2016 Jun 1. Anal Biochem. 2017. PMID: 27261176 Free PMC article.