Deoxyribozyme-based half-adder

Milan N Stojanović; Darko Stefanović

doi:10.1021/ja0296632

Deoxyribozyme-based half-adder

J Am Chem Soc. 2003 Jun 4;125(22):6673-6. doi: 10.1021/ja0296632.

Authors

Milan N Stojanović¹, Darko Stefanović

Affiliation

¹ Division of Clinical Pharmacology and Experimental Therapeutics, Department of Medicine, Columbia University, Box 84, 630 W 168th Street, New York, NY 10032, USA. mns18@columbia.edu

PMID: 12769576
DOI: 10.1021/ja0296632

Abstract

We have constructed a solution-phase array of three deoxyribozyme-based logic gates that behaves as a half-adder. Two deoxyribozymes mimic i(1)ANDNOTi(2) and i(2)ANDNOTi(1) gates that cleave a fluorogenic substrate, reporting through an increase in fluorescence emission at 570 nm. The third deoxyribozyme mimics an i(1)ANDi(2) gate and cleaves the other fluorogenic substrate, reporting through an increase in fluorescence emission at 520 nm. Together, this system represents the first example of a decision-making enzymatic network with two inputs and two outputs. Similar systems could be applied to control autonomous therapeutic and diagnostic devices.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Sequence
DNA, Catalytic / chemistry*
Logic*
Molecular Sequence Data
Nucleic Acid Conformation
Spectrometry, Fluorescence

Substances

DNA, Catalytic

Grants and funding

R01 EB 000675-1/EB/NIBIB NIH HHS/United States